Cellular and molecular basis of cadmium‐induced deformities in zebrafish embryos
Identifieur interne : 000D97 ( Main/Exploration ); précédent : 000D96; suivant : 000D98Cellular and molecular basis of cadmium‐induced deformities in zebrafish embryos
Auteurs : Shuk Han Cheng [République populaire de Chine] ; Albert Wing Kong Wai [République populaire de Chine] ; Chun Hung So [République populaire de Chine] ; Rudolf Shiu Sun Wu [République populaire de Chine]Source :
- Environmental Toxicology and Chemistry [ 0730-7268 ] ; 2000-12.
Descripteurs français
- Wicri :
- topic : Cadmium.
English descriptors
- KwdEn :
- Teeft :
- Abnormality, Axial, Axial curvature, Brachydanio rerio, Cadmium, Cadmium concentration, Cadmium concentrations, Cadmium embryos, Cadmium teratogenesis, Cadmium treatment group, Cardiac, Cardiac edema, Caudal, Caudal duplication, Chem, City university, Control embryos, Control group, Curvature, Danio rerio, Dead embryos, Deformity, Developmental abnormalities, Developmental events, Developmental toxicity, Difference test, Early neurogenesis, Ectopic expression, Elbe estuary, Embryo, Embryonic, Embryonic development, Embryonic malformations, Environ, Eve1, Fertilization, Genetic pathways, Hedgehog, Hong kong, Hypoplasia, Inductive signals, Malformation, Malformed embryos, Marker genes, Median effect concentration, Model system, Molecular basis, Molecular events, Molecular mechanisms, Musculature development, Myosin, Myotome formation, Neural, Neural development, Neural tissue, Neural tissues, Neural tube, Polychlorinated biphenyls, Positive control, Posterior cells, Present study, Previous studies, Room temperature, Scoliotic, Scoliotic embryos, Skeletal deformities, Software, Software toxcalc, Somite, Somite patterning, Sonic, Sonic hedgehog, Spinal deformities, Standard error, Statistical analyses, Tail extension, Tail malformations, Tail tips, Teratogenesis, Test solutions, Tidepool software, Toxicity, Toxicol, Treatment concentrations, Untreated control, Untreated controls, Vertical bars, Xenopus laevis, Yolk.
Abstract
Cadmium is known to cause developmental defects in a varietyof vertebrate species, but relatively little is known about the underlying molecular mechanisms. In this study, we used zebrafish (Danio rerio) embryos as a model system to investigate cadmium‐induced toxicities. Fertilized embryos collected at 5‐h after fertilization were incubated for 18 h in culture media containing 1 to 1, 000 μM CdCl2. The median embryolethal concentration (LC50) was 168 μM, whereas the median effect concentration (EC50) for total adverse effect (mortality and developmental defects) was 138 μM. Six major types of deformities were observed: head and eye hypoplasia, hypopigmentation, cardiac edema, yolk sac abnormalities, altered axial curvature, and tail malformations. The frequency of malformations increased with cadmium concentration. Somites of embryos with altered axial curvature were investigated using the antimyosin antibody MF‐20. This study demonstrated, to our knowledge for the first time, reduced myotome formation in cadmium‐induced spinal deformity. Embryos with head and eye hypoplasia were studied using the anti‐neural tissue antibody zns‐2, and a poorly developed central nervous system was revealed. Head and eye hypoplasia were associated with lack of expression of the sonic hedgehog gene, which controls the patterning of the neural tube and somites. Genes involved in tail formations, such as evenskipped 1 and no tail, were ectopically expressed in embryos with tail malformations. Our data support the hypothesis that fish embryonic malformations induced by cadmium might be mediated through ectopic expression of developmental regulatory genes.
Url:
DOI: 10.1002/etc.5620191223
Affiliations:
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Le document en format XML
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<term>Sonic hedgehog</term>
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<term>Axial</term>
<term>Axial curvature</term>
<term>Brachydanio rerio</term>
<term>Cadmium</term>
<term>Cadmium concentration</term>
<term>Cadmium concentrations</term>
<term>Cadmium embryos</term>
<term>Cadmium teratogenesis</term>
<term>Cadmium treatment group</term>
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<term>Cardiac edema</term>
<term>Caudal</term>
<term>Caudal duplication</term>
<term>Chem</term>
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<term>Control group</term>
<term>Curvature</term>
<term>Danio rerio</term>
<term>Dead embryos</term>
<term>Deformity</term>
<term>Developmental abnormalities</term>
<term>Developmental events</term>
<term>Developmental toxicity</term>
<term>Difference test</term>
<term>Early neurogenesis</term>
<term>Ectopic expression</term>
<term>Elbe estuary</term>
<term>Embryo</term>
<term>Embryonic</term>
<term>Embryonic development</term>
<term>Embryonic malformations</term>
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<term>Eve1</term>
<term>Fertilization</term>
<term>Genetic pathways</term>
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<term>Hong kong</term>
<term>Hypoplasia</term>
<term>Inductive signals</term>
<term>Malformation</term>
<term>Malformed embryos</term>
<term>Marker genes</term>
<term>Median effect concentration</term>
<term>Model system</term>
<term>Molecular basis</term>
<term>Molecular events</term>
<term>Molecular mechanisms</term>
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<term>Myotome formation</term>
<term>Neural</term>
<term>Neural development</term>
<term>Neural tissue</term>
<term>Neural tissues</term>
<term>Neural tube</term>
<term>Polychlorinated biphenyls</term>
<term>Positive control</term>
<term>Posterior cells</term>
<term>Present study</term>
<term>Previous studies</term>
<term>Room temperature</term>
<term>Scoliotic</term>
<term>Scoliotic embryos</term>
<term>Skeletal deformities</term>
<term>Software</term>
<term>Software toxcalc</term>
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<term>Somite patterning</term>
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<term>Standard error</term>
<term>Statistical analyses</term>
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<term>Tail malformations</term>
<term>Tail tips</term>
<term>Teratogenesis</term>
<term>Test solutions</term>
<term>Tidepool software</term>
<term>Toxicity</term>
<term>Toxicol</term>
<term>Treatment concentrations</term>
<term>Untreated control</term>
<term>Untreated controls</term>
<term>Vertical bars</term>
<term>Xenopus laevis</term>
<term>Yolk</term>
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<front><div type="abstract" xml:lang="en">Cadmium is known to cause developmental defects in a varietyof vertebrate species, but relatively little is known about the underlying molecular mechanisms. In this study, we used zebrafish (Danio rerio) embryos as a model system to investigate cadmium‐induced toxicities. Fertilized embryos collected at 5‐h after fertilization were incubated for 18 h in culture media containing 1 to 1, 000 μM CdCl2. The median embryolethal concentration (LC50) was 168 μM, whereas the median effect concentration (EC50) for total adverse effect (mortality and developmental defects) was 138 μM. Six major types of deformities were observed: head and eye hypoplasia, hypopigmentation, cardiac edema, yolk sac abnormalities, altered axial curvature, and tail malformations. The frequency of malformations increased with cadmium concentration. Somites of embryos with altered axial curvature were investigated using the antimyosin antibody MF‐20. This study demonstrated, to our knowledge for the first time, reduced myotome formation in cadmium‐induced spinal deformity. Embryos with head and eye hypoplasia were studied using the anti‐neural tissue antibody zns‐2, and a poorly developed central nervous system was revealed. Head and eye hypoplasia were associated with lack of expression of the sonic hedgehog gene, which controls the patterning of the neural tube and somites. Genes involved in tail formations, such as evenskipped 1 and no tail, were ectopically expressed in embryos with tail malformations. Our data support the hypothesis that fish embryonic malformations induced by cadmium might be mediated through ectopic expression of developmental regulatory genes.</div>
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